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Pyruvate kinase isoenzymes: L-PK
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L-PK staining of the liver of a healthy rat
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Counterstaining with M2-PK specific antibodies
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A homogeneous staining of the liver parenchyma cells is detectable. The Trias with portal vein, artaie and bile duct are not L-PK positive.
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The liver parenchyma cells are not stained with the M2-PK specific antibody. The bile duct area and the endothel of the arteria are M2-PK positive.
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Protocol for immunostaining
References
Pyruvate Kinase isoenzymes
Glycolysis : isoenzyme equipment
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