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Glycolytic enzyme complex: isolation
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The glycolytic enzyme complex can be demonstrated by isoelectric focusing:
Proteins that are associated inside the glycolytic enzyme complex focuse at a common isoelectric point that is different from the isoelectric points of the purified individual proteins.
RNAse treatment leads to a disruption of the complex which is reflected by a dispersion of the isoelectric points of the individual proteins.
Data
Transformation is correlated with migrations of proteins in- and outside the glycolytic enzyme complex that are reflected by shifts in the isoelectric points of the proteins.
Function of the glycolytic enzyme complex
References
Composition of the glycolytic enzyme complex
Glycolysis : main page
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