Staining of a rectal carcinoma

with monoclonal antibodies against Tumor M2-PK

The normal rectal mucosa is not stained with the Tumor M2-PK specific antibody whereas the carcinoma is Tumor M2-PK positive.

The Tumor M2-PK staining of primary tumors is usually heterogeneous whereas their metastases are always very homogeneously stained.

Staining of a liver metastasis
from rectal carcinoma

with monoclonal antibodies against tumor M2-PK

Vmax activities were measured spectrophotometrically according to the following reaction:

Tumor M2-PK measurements in stool and EDTA-plasma samples:
Hans-Ulrich Klör
Philip D. Hardt

Tumor M2-PK concentrations were measured with a sandwich ELISA from ScheBo Biotech AG Giessen, Germany.

References

Tumor M2-PK: clinical relevance for metabolic profiling of tumors

Metabolic tools for tumor diagnosis

Pyruvate kinase isoenzymes: M2-PK