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Pyruvate kinase type M2: amino acid sequence
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The M2 and M1-PK isoenzymes are encoded by the same gene but result from alternative splicing of exons 9 and 10. The human M2 and M1-PK isoenzyme differ in 23 amino acids within a stretch of 56 amino acids.
Amino acid sequence of human pyruvate kinase type M2
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56 amino acids (379 - 435) differing between M1 and M2-PK
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underlined
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phosphorylation sites: tyrosine (390); threonine (405), serine (406)
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Fructose 1,6-P2 binding
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Lys 433, Ser 434, Ser 437, Gly 520 (6´-phoshate oxygen)
Arg 489, Gly 518, Trp 482 (1´-phosphate)
Phe 521, Gly 514, (O2´-and O4´hydroxyls)
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underlined
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HERC1-interacting region (407-531)
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active site (Lys 270, Lys 367)
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Comparison of human M2-PK and M1-PK sequences
References
Pyruvate kinase isoenzymes : M2-PK
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